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CAMEL REPRODUCTION |
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Scientific Staff : Dr. Aminudeen, Dr. Sumant Vyas, Dr. Gorakh Mal
Project : Improving reproductive efficiency in camel |
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Sub projects |
- Application of artificial insemination in dromedarius camel
- Superovulation and embryo collection from female camels
- Application of sonography and other diagnostic techniques in fertility and infertility management
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Research Achievements |
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Radio-immuno assay (RIA) for quantification of Estradiol-17β has been standardized using anti-oestradiol and 2,4,6,7,16,17-3H Oestradiol as tracer. It has been found that the low ambient temperature with the onset of winter disturbed radioimmunoassay due to increased association constant of antibody. While facing this problem, an important aspect of antibody-heptanes interaction was noticed that the low temperature increases association constant of antibody. RIA based on liquid scintillation tritiated labeled tracer may also get disturbed due to degeneration of tracer while handling.
- Anti-ovine LH rabbit serum has been raised and tested through double immuno- diffusion test.
- Anti-rabbit gamma globulin was successfully raised in sheep and tested through double immuno- diffusion test.
- Estradiol and progesterone profiles of female camels during different stages of reproduction have been assayed. Average progesterone levels in pregnant camels were found to be around 0.5 ng/ml from day 0 to day 3, it rises to 0.7-0.85 ng/ml on day 4-5, above 1ng/ml on day 6, above 2ng/ml on day 9, above 3ng/ml on day 13 and above 4ng/ml on day 28. Average progesterone levels were similar in non-pregnant animals to those of pregnant up to day 11. From day 12 onwards, the progesterone levels declined rapidly.
- Testosterone profiles and sexual libido has been recorded in Jaisalmeri camels at monthly intervals through out the year. The average Testosterone concentrations were low during hot months (April to September). Testosterone concentration increased in the month of October, November and December which peaked in the month of January and February. However it started declining in the month of March to low levels in the months to follow.
- Copulation time(s) has been found high during January to April while it declined slowly over May and complete cessation of libido was observed during later half of June. Correlation coefficient values for T, copulation time and volume of semen (r= 0.847, 0.798 and 0.957, respectively) showed positive correlation among endocrine and sexual libido parameters.
- Camel semen does not exhibit mass motility due to entrapment of spermatozoa. It does not mix with physiological buffers and egg yolk based tris buffer extenders.
- Semen cryopreservation was accomplished with 70 semen samples artificially collected from 11 adult male camels. The artificial impregnation of female camels with cryopreserved semen was not successful.
- Superovulation was attempted using progesterone ear implant, PMSG and hCG treatment in female camels. However the results were not favorable due to the poor superovulatory response.
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